Tri- and tetravalent mannoclusters cross-link and aggregate BC2L-A lectin from Burkholderia cenocepacia

Csávás, M., Malinovská, L., Perret, F., Gyurkó, M., Illyés, T. Z., Wimmerová, M., Borbás, A.: Tri- and tetravalent mannoclusters cross-link and aggregate BC2L-A lectin from Burkholderia cenocepacia.
Carbohydr. Res. 437, 1-8, 2017.

doi DEA WoS Scopus

Journal metrics:

Q2 Analytical Chemistry

Q3 Biochemistry

Q2 Medicine (miscellaneous)

Q2 Organic Chemistry

title:

Tri- and tetravalent mannoclusters cross-link and aggregate BC2L-A lectin from Burkholderia cenocepacia

authors:

Csávás Magdolna
Malinovská, Lenka
Perret, Florent
Gyurkó Milán
Illyés Tünde Zita
Wimmerová, Michaela
Borbás Anikó

corresponding author:

Borbás Anikó

published:

2017

type:

article

genre:

research article/review article

journal:

Carbohydrate Research (ISSN: 0008-6215)

language:

English

HAC:

Natural Sciences, Chemical Sciences

subjects:

Anti-adhesion therapy, Click reaction, Glycoclusters, Mannose-binding lectin, Burkholderia cenocepacia

abstract:

The opportunistic Gram-negative bacterium Burkholderia cenocepacia causes lethal infections in cysticfibrosis patients. Multivalent mannoside derivatives were prepared as potential inhibitors of lectin BC2LA,one of the virulence factors deployed by B. cenocepacia in the infection process. An (a1/2)-thiolinkedmannobioside mimic bearing an azide functionalized aglycon was conjugated to differentmultivalent scaffolds such as propargylated calix[4]arenes, methyl gallate and pentaerythritol by azidealkyne1,3-dipolar cycloaddition. The interaction between the glycoclusters and the mannose bindingBC2L-A lectin from B. cenocepacia was examined by isothermal microcalorimetry, surface plasmonresonance, inhibition of yeast agglutination and analytical ultracentrifugation.

projects:

NKFIH K119509; OTKA-109208